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Updated: Jul 17, 2026

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
Published on: September 6, 2018
Discrete stimulatory effects of platelet-derived growth factor (PDGF-BB) on Leydig cell steroidogenesis
1Institute of Reproduction & Development, Monash University, Monash Medical Centre, Clayton, Victoria, Australia.
Abstract:
The effect of platelet-derived growth factor-BB on adult rat Leydig cell steroidogenesis has been determined using culture conditions which maintain testosterone production and responsiveness to luteinising hormone for 3 days. A significant stimulation of testosterone levels was observed in acute response to a maximally stimulating dose (8 ng/ml) of rat luteinising hormone on the third day of culture, but was dependent on pre-exposure of the Leydig cells to PDGF-BB for 2 days, whilst maintained in the presence of a minimum dose (0.1 ng/ml) of rat luteinising hormone. These data demonstrate a very discrete action of PDGF-BB on adult rat Leydig cell steroidogenesis in vitro, but whether or not this constitutes a significant paracrine effect in vivo remains to be established.
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