Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Sheep thyroxine-binding globulin: cDNA sequence and expression

A Tsykin¹, G Schreiber

¹Russell Grimwade School of Biochemistry, University of Melbourne, Australia.

Molecular and Cellular Endocrinology

|December 1, 1993

Summary

Researchers isolated a sheep thyroxine-binding globulin (TBG) clone, revealing high similarity to human and rat sequences. TBG gene expression was primarily hepatic and present early in sheep fetal development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The global transcriptomic signature in sinonasal tissues reveals roles for tissue type and chronic rhinosinusitis disease phenotype.

Rhinology·2020

Same author

Pre-therapy mRNA expression of TNF is associated with regimen-related gastrointestinal toxicity in patients with esophageal cancer: a pilot study.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2015

Same author

Quantitative cytology research; the rhythmic growth of the nucleus in the renal channels of Leptodactylus; conditions on somatic polyploidism.

Revista brasileira de biologia·2014

Same author

Enhanced expression of transferrin receptor 1 contributes to oncogenic signalling by sphingosine kinase 1.

Oncogene·2013

Same author

The Archaebacterium Thermofilum pendens Represents, a Novel Genus of the Thermophilic, Anaerobic Sulfur Respiring Thermoproteales.

Systematic and applied microbiology·2012

Same author

Typha capensis (Rohrb.)N.E.Br. (bulrush) extract scavenges free radicals, inhibits collagenase activity and affects human sperm motility and mitochondrial membrane potential in vitro: a pilot study.

Andrologia·2011

Area of Science:

Molecular Biology
Genetics
Endocrinology

Background:

Thyroxine-binding globulin (TBG) is crucial for thyroid hormone transport.
Understanding TBG's molecular characteristics and expression is vital for comparative studies.

Purpose of the Study:

To isolate and characterize the sheep thyroxine-binding globulin (TBG) gene.
To investigate the tissue-specific and developmental expression of the TBG gene in sheep.

Main Methods:

cDNA library screening to isolate the TBG clone.
Nucleotide sequencing to determine gene structure.
Northern blot analysis to assess gene expression patterns.

Main Results:

A full-length sheep TBG clone was obtained, with a mature protein of 396 amino acids.

Related Experiment Videos

Sheep TBG sequence showed 80% and 76% similarity to human and rat TBG, respectively.

TBG gene expression was exclusively detected in the liver, with detectable mRNA in early fetal development.

Conclusions:

The sheep TBG gene is conserved across species and primarily expressed in the liver.
Hepatic TBG expression is established early in ovine fetal development, reaching adult levels mid-pregnancy.