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Nucleotide sequence of the bovine vimentin-encoding cDNA
J F Hess1, J T Casselman, P G FitzGerald
1Department of Cell Biology and Human Anatomy, School of Medicine, University of California 95616.
Gene
|March 25, 1994
Summary
Researchers amplified and sequenced bovine lens vimentin (Vim) using RT-PCR. The bovine Vim amino acid sequence shows high homology to human and mouse Vim, indicating conserved structure.
Area of Science:
- Biochemistry
- Molecular Biology
- Ophthalmology
Background:
- Vimentin (Vim) is a crucial intermediate filament protein.
- Lens proteins are vital for maintaining ocular clarity and function.
Purpose of the Study:
- To amplify and characterize the vimentin (Vim) encoding sequences from bovine lens RNA.
- To determine the amino acid sequence homology of bovine vimentin with other species.
Main Methods:
- Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify Vim sequences.
- Amplified products were cloned and nucleotide sequences were determined.
Main Results:
- Vimentin (Vim) encoding sequences were successfully amplified from bovine lens RNA.
- The deduced amino acid sequence of bovine vimentin exhibited high homology (97.2%) to human vimentin.
- Significant homology was also observed with mouse (96.8%), hamster (96.3%), and chicken (84.9%) vimentin.
Conclusions:
- The study successfully characterized bovine lens vimentin.
- Bovine vimentin shares high sequence homology with mammalian and avian vimentin, suggesting conserved structural and functional roles across species.