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The murine glutamine:fructose-6-phosphate amidotransferase-encoding cDNA sequence

P P Sayeski¹, A J Paterson, J E Kudlow

¹Department of Medicine, University of Alabama at Birmingham 35294.

|March 25, 1994

Summary

Researchers cloned mouse Glutamine:fructose-6-phosphate amidotransferase (GFAT) cDNA, finding high homology to human GFAT. Mouse GFAT is encoded by a 7-kb mRNA, unlike the multiple human mRNA sizes.

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Area of Science:

Biochemistry
Molecular Biology
Genetics

Background:

Glutamine:fructose-6-phosphate amidotransferase (GFAT) is the key regulatory enzyme in the hexosamine biosynthesis pathway.
GFAT plays a crucial role in controlling growth factor gene expression.
Understanding GFAT's molecular characteristics is vital for metabolic and cellular regulation research.

Purpose of the Study:

To clone and characterize the mouse cDNA encoding Glutamine:fructose-6-phosphate amidotransferase (GFAT).
To compare the mouse GFAT sequence with its human counterpart.
To investigate the mRNA expression patterns of GFAT in mice versus humans.

Main Methods:

Cloning of mouse cDNA for GFAT.
Sequence analysis and comparison between mouse and human GFAT.

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Analysis of mRNA size and expression using techniques like Northern blotting (implied).

Main Results:

A mouse cDNA sequence for GFAT was successfully cloned.
The mouse GFAT cDNA exhibits 91% homology to the human sequence.
The deduced amino acid sequence of mouse GFAT shows 98.6% identity to human GFAT.
Mouse GFAT is transcribed from a single 7-kb mRNA, contrasting with multiple human mRNA sizes.

Conclusions:

The high sequence identity suggests conserved function between mouse and human GFAT.
Differences in mRNA processing or transcription initiation exist between species.
This study provides a foundation for further research into GFAT's role in mammalian systems.