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CDw60: a marker for human CD8+ T helper cells

E P Rieber1, G Rank

  • 1Institute for Immunology, University of Munich, Germany.

The Journal of Experimental Medicine
|April 1, 1994
PubMed
Summary

Researchers identified the CDw60 antigen on CD8+ T cells, distinguishing helper and cytotoxic/suppressor functions. This discovery aids in understanding immune cell roles and developing targeted therapies.

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Area of Science:

  • Immunology
  • Cell Biology
  • T cell subsets

Background:

  • The CD8+ T cell subset includes cells with helper functions, but their specific phenotype has been unclear.
  • Distinguishing between CD8+ T helper cells and those with cytotoxic/suppressor roles is crucial for immune response understanding.

Purpose of the Study:

  • To identify a specific cell surface marker that distinguishes CD8+ T helper cells from CD8+ T cells with cytotoxic and suppressor functions.
  • To characterize the functional properties of CD8+ T cell subsets defined by CDw60 expression.

Main Methods:

  • Utilized CDw60 monoclonal antibodies (mAb) to identify the CDw60 antigen on human CD8+ T cells.
  • Employed mAb-rosetting techniques for high-purity isolation of CDw60+CD8+ and CDw60-CD8+ cell populations.
  • Assayed cytotoxic activity, B cell helper/suppressor functions, and cytokine production (IL-2, IL-4, interferon-gamma) of isolated subsets.

Main Results:

  • CDw60+CD8+ cells exhibited helper activity for B cell differentiation, while CDw60-CD8+ cells showed suppressor capacity.
  • Alloantigen-specific cytotoxic activity was exclusively found in the CDw60-CD8+ population.
  • Both subsets produced IL-2; IL-4 was preferentially secreted by CDw60+CD8+ cells, and interferon-gamma by CDw60-CD8+ cells.

Conclusions:

  • CDw60 expression on human CD8+ T cells serves as a reliable marker to differentiate between T helper and T cytotoxic/suppressor subsets.
  • The CDw60 marker, in conjunction with others, can define CD8+ T cell subsets with distinct and reciprocal immune functions.

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