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Updated: Oct 9, 2026

Harvesting Sperm and Artificial Insemination of Mice
Published on: April 27, 2007
Optimizing semen production for artificial insemination in swine
B Colenbrander1, H Feitsma, H J Grooten
1Cooperative Pig AI-Centre, Vught, The Netherlands.
Abstract:
Efficient production of high quality semen is of major importance to artificial insemination (AI) organizations. The semen produced should be free of contagious organisms, be of high quality, have good storage properties, fertilizing capacity and be of high genetic value. The best approach to prevent the spreading of microorganisms via semen in the process of AI is to collect semen from boars free from specific diseases, for example pseudorabies virus or leptospirosis. Antibiotics are added to the semen to suppress proliferation of microorganisms or even reduce their number. Sperm production is influenced by many factors such as season, collection frequency, breed and age. The average number of sperm cells produced per boar per week can vary more than 30% within one AI station, depending on the breed. Boar selection and boar management markedly influence the efficiency of sperm production. Sperm quality should be evaluated by fertility results obtained at breeding farms related to both farrowing rate and litter size to ensure a good quality monitoring system. A quality control system should be established to provide maximum reliability to customers.

