[Study of segments of 23S RNA, important for interaction with elongation factors Tu and G using complementary...

Several oligonucleotides complementary to different 23S RNA regions were tested in the elongation factor-dependent reactions of the ribosomes. It was found that the 1088-1100 and 1127-1140 sequence parts of the 23S RNA (binding regions for the L11 protein) are very important for EF-G function. The EF-Tu function is markedly less affected by these nucleotides. The probable role of 23S RNA function is discussed.