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Multi-target PCR analysis by capillary electrophoresis and laser-induced fluorescence
1Laboratory of Tumor Immunology, Hopital Laennec, Université de Paris V, France.
Nature
|March 17, 1994
Abstract:
Quantitative analysis of polymerase chain reaction (PCR) amplified HIV-1 DNA or cDNA fragments is attained using an automated system that combines capillary-gel electrophoresis (CGE) for high-efficiency separation and laser-induced fluorescence (LIF) for high-sensitivity detection. This system enables the detection of PCR-amplified multiple target DNA or cDNA in the same tube by a single injection with high precision.