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Neutral alpha-mannosidase activity in human serum

B Hultberg, P K Masson, S Sjöblad

    Biochimica Et Biophysica Acta
    |September 14, 1976
    PubMed
    Summary

    Serum contains two types of alpha-mannosidase (alpha-D-mannoside mannohydrolase) with neutral pH optima. These distinct enzyme forms can be identified using various chromatographic methods and have implications for understanding mannosidosis.

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    Area of Science:

    • Biochemistry
    • Enzymology
    • Molecular Biology

    Background:

    • Serum contains alpha-mannosidase (alpha-D-mannoside mannohydrolase, EC 3.2.1.24) activity.
    • Two distinct neutral pH optima activities for alpha-mannosidase have been identified in serum.

    Purpose of the Study:

    • To characterize and differentiate the two neutral alpha-mannosidase activities in serum.
    • To investigate the relationship between serum alpha-mannosidase forms and mannosidosis.

    Main Methods:

    • Gel-filtration chromatography
    • DEAE-cellulose chromatography
    • Concanavalin-A Sepharose chromatography
    • Enzyme activity assays under varying pH, temperature, and substrate inhibition conditions.

    Main Results:

    • Two alpha-mannosidase activities with neutral pH optima were confirmed in serum.
    • One activity (pH 6.0-6.4) resembles tissue alpha-mannosidase C.
    • The dominant serum activity has a pH optimum between 5.2 and 5.8.
    • Chromatographic methods differentiated these forms, with serum-type neutral activity co-eluting with acidic forms.
    • Distinct responses to pH, heating, and methyl-alpha-D-mannopyranoside inhibition further distinguished the two activities.

    Conclusions:

    • Serum possesses at least two distinct alpha-mannosidase forms with neutral pH optima.
    • These forms exhibit unique biochemical properties allowing for their differentiation.
    • Understanding these serum alpha-mannosidase activities is crucial for research into lysosomal storage diseases like mannosidosis.

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