Requirement of the laying hen for sulfur amino acids

Two experiments with individually caged laying hens were conducted to determine the requirement for TSAA. In Experiment 1, the corn-soybean basal diet contained .48% TSAA to which increasing dose levels of DL-methionine were added providing at the highest supplemental level .645% TSAA. The experimental diets were fed for 12 wk, covering the early stage of laying from 25 to 37 wk of age. In Experiment 2, a corn-soybean basal diet containing of .51% TSAA was used along with DL-methionine supplements to determine TSAA requirement during the entire laying cycle of 52 wk (25 to 77 wk of age). The highest supplemental level of DL-methionine in this experiment provided .76% TSAA. The TSAA requirement was found to be higher for maximum efficiency of feed utilization than for obtaining maximum egg production. Based on feed conversion efficiency and at an egg mass yield of 55 g per hen-day, the requirement for TSAA was estimated to be about 740 mg per hen-day, of which about 440 mg was methionine, throughout a laying period of 52 wk. It was calculated that the estimated TSAA requirement was equivalent to approximately 660 mg true digestible SAA per hen-day.