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Related Experiment Videos

C3 polymorphism in polyacrylamide-gel electrophoresis

J Schwamborn, H Freis, G Mauff

    Vox Sanguinis
    |January 1, 1976
    PubMed
    Summary

    Complement component 3 (C3) polymorphism was identified using crossed immunoelectrophoresis. Rare C3 phenotypes were observed in native C3 and its conversion product, C3c.

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    Human genetics·1997

    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • Complement component 3 (C3) is a crucial protein in the immune system.
    • Understanding C3 polymorphism is important for immunological studies.

    Purpose of the Study:

    • To identify and characterize C3 polymorphism using polyacrylamide-gel electrophoresis.
    • To investigate C3 polymorphism in native C3 and its conversion products.

    Main Methods:

    • Crossed immunoelectrophoresis was employed to detect C3 polymorphism.
    • Sera were treated with cobra venom factor, endotoxin, and neuraminidase to analyze C3 conversion products.

    Main Results:

    • C3 polymorphism was successfully identified in native C3.
    • Polymorphic bands were also observed in C3c, a C3 conversion product, after various treatments.
    • Rare C3 phenotypes were detectable in both native C3 and C3c.

    Conclusions:

    • Crossed immunoelectrophoresis is a reliable method for identifying C3 polymorphism.
    • C3 polymorphism extends to its conversion product, C3c.
    • The study highlights the presence of rare C3 phenotypes.

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