Peripheral proprioceptive modulation in crayfish walking leg by serotonin

In vitro serotoninergic modulation of intracellularly recorded sensory responses was examined in primary afferent terminals of a crayfish leg proprioceptor, the coxo-basal chordotonal organ (CB CO). The effects of different concentrations of serotonin (5-HT) on static and dynamic sensory responses were analysed following bath or pressure applications of the monoamine directly on the strand of the mechanoreceptor. Consequently, the reported effects result from the direct peripheral action of 5-HT on the sensory organ itself. Serotonin modulates the sensory activity by modifying the sensory discharge frequency. The firing discharge of the primary afferents is increased in a dose-dependent manner. The maximal effect is obtained with a concentration of 10(-6) M. Higher concentrations are less effective, and for 20% of the recorded cells, 10(-4) M 5-HT induces a decrease of the sensory discharge, i.e. has an inhibitory effect. Alteration in the pattern of sensory firing, resulting in bursting discharge, was observed in some units. All the recorded sensory units were responsive to the neuromodulator whatever their functional properties. The effects of 5-HT lasted as long as the amine was applied and were reversible after wash. The results suggest that 5-HT could exert a modulatory action on the proprioceptive feedback, by peripheral action on the sensory organ. The natural modalities of 5-HT action are discussed on the basis of immunohistochemistry data suggesting: (i) connections between CB CO and central serotoninergic cells, (ii) 5-HT content in sensory cells of the CB CO. Since the CB CO is involved in the control of leg movement and position, the modulation of its primary afferents might influence the organization of the locomotor pattern. The functional significance of this peripheral sensory neuromodulation was approached by the analysis of the motor reflex activity.