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In human chromosomes telomeric regions are enriched in CpGs relative to R-bands
M Ferraro1, V Predazzi, G Prantera
1Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Roma, Italy.
Chromosoma
|December 1, 1993
Abstract:
Human chromosomes were in situ nick-translated using as nicking agents the endonucleases MspI (CCGG), its methyl-sensitive isoschizomer HpaII, HaeIII (GGCC), SacII (CCGCGG), EcoRI (GAATTC) and DNaseI. We show that in metaphase chromosomes R-bands are enriched, as compared with G-bands, in the dinucleotide CpG but no more than what is expected on the basis of their relative G+C content. The telomeric regions, on the contrary, besides having a chromatin conformation that is particularly relaxed and accessible to endonucleases, also show an enrichment in CpGs.