Distribution of actin in developing outer hair cells in the gerbil

During the two weeks following the onset of cochlear function in the gerbil, active cochlear processes appear to mature. The active cochlear processes likely involve outer hair cells with their specialized lateral wall structures, including the subsurface cisternae and associated cytoskeletal elements. We have previously demonstrated that the subsurface cisternae mature gradually during the time that active cochlear processes mature in the gerbil. In the study reported here, we used postembedding immunocytochemical electron microscopy to investigate whether actin labelling associated with the cortical cytoskeleton of the gerbil outer hair cell increased concomitantly. In contrast to the gradual development of the subsurface cisternae, actin labelling in the region of the cortical cytoskeleton significantly increased during the onset of cochlear function and maintained this level during the time that active cochlear processes mature. Thus, it appears that increased actin adjacent to the lateral plasma membrane of the outer hair cell is related to the onset of cochlear function rather than to the maturation of active cochlear processes.