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Updated: Aug 10, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
[The effect of the ascorbic acid-Cu (II) couple on enzyme activity]
1Disciplina de Biochimie, Universitatea de Medicină şi Farmacie Iaşi.
Abstract:
Ascorbic acid (AA), Cu (II) and their association affect in different ways the activity of G6P-ase and G6PDH from the rat hepatic cytoplasm enriched in microsomes. G6P-ase is inhibited at a wide range of concentrations: 200-2 mM AA and 1-0.1 mM Cu (II). The association AA-Cu in the above mentioned concentrations, proved to be a stronger inhibitor than AA or Cu (II) introduced separately in the incubation medium. The activity of G6PDH is stimulated by AA-Cu (II) association and inhibited by AA in the dialyzed and nondialyzed cytoplasm. Cu (II) inhibits the activity of G6PDH in the nondialyzed cytoplasm and stimulates it following dialysis. The compounds with recognized antiradical action prove to be inefficient in restoring the activity of G6PDH affected by the AA-Cu (II) association.
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