The effect of highly diluted agitated thyroxine on the climbing activity of frogs

We studied the influence of specially prepared highly diluted thyroxine on the spontaneous tendency of juvenile frogs, which were at the end of thyroxine-controlled metamorphosis, to leave the water and climb onto land. The test dilution with a thyroxine concentration beyond Avogadro's value (dilution thyroxine D30) and the reference (dilution water D30) were prepared according to directions from the literature on homeopathy. A few drops of these solutions were added to tap water of basins containing the frogs. The frogs' climbing activities were monitored immediately after adding the solutions. The hypothesis derived from a preliminary study was that there is less climbing activity in frogs treated with dilution thyroxine D30 than in a reference group. This hypothesis was proven. Climbing activity diminished under the influence of dilution thyroxine D30, with statistical significance both in comparison to the effect of the analogously prepared solvent (dilution water D30) as well as in comparison to control observations before the start of treatment. When in a later step of observation the dilution water D30-control group was treated with dilution thyroxine D30, the diminishing effect on activity also occurred.