[Effectiveness of proteolytic enzymes]
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Updated: Nov 9, 2026

Detection of Protease Activity by Fluorescent Peptide Zymography
Published on: January 20, 2019
Abstract:
A theory of the ground state's destabilization is developed explaining the high efficiency of proteases. The theory suggests a participation of the valence interactions in the formation of a productive complex and connects the efficiency and the specificity of proteolysis with the structure of such a complex. Proved is nonproductivity of nonvalence complexes in which the reactive amide group of the substrate is planar. It is suggested that the theory of the absolute rates of reactions does not cover the process of the enzymatic hydrolysis.
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