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Developmental changes of renal brushborder membrane ionic permeability

M S Medow1, M S Lipkowitz

  • 1Department of Pediatrics, New York Medical College, Valhalla 10595.

Biochimica Et Biophysica Acta
|April 20, 1994
PubMed
Summary
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Renal brush-border membrane vesicles (BBMV) exhibit age-related changes in ion permeability and lipid fluidity. These alterations in sodium and potassium permeability influence solute transport in developing kidneys.

Area of Science:

  • Nephrology
  • Membrane Transport
  • Developmental Biology

Background:

  • Renal brush-border membrane vesicles (BBMV) display age-dependent variations in solute transport.
  • Early studies noted increased Na(+)-gradient driven overshoot for glucose and proline, and faster 22Na+ uptake in younger rats.

Purpose of the Study:

  • To investigate the underlying mechanisms of age-dependent changes in renal BBMV solute transport.
  • To characterize the ionic permeability and lipid properties of BBMV from rats of different ages.

Main Methods:

  • Utilized a voltage-sensitive fluorescent dye (diS-C3(5)) to assess ionic permeability of BBMV.
  • Determined absolute and relative ionic permeabilities (K+, Cl-, Na+) after incubation in KCl.
  • Employed fluorescence polarization to analyze lipid fluidity of BBMV from different aged rats.

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Main Results:

  • Intravesicular potassium ([K+]in) was significantly lower in 7-day-old rats compared to adults.
  • The ratio of sodium to potassium permeability (PNa+/PK+) decreased significantly with age.
  • Adult BBMV exhibited lower PNa+ relative to PCl-, enhancing Na(+)-solute co-transport under NaCl gradients.
  • Lipids in BBMV from younger rats were more fluid than those from adults.

Conclusions:

  • Developmental changes in lipid composition influence renal BBMV membrane transport and permeability.
  • Altered ionic permeability and lipid fluidity contribute to age-dependent modifications in renal BBMV solute transport.
  • These findings provide insights into the physiological adaptations of the developing kidney.