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Does phosphorylation affect transport of inorganic phosphate?
Clinical and Experimental Pharmacology & Physiology
|January 1, 1994
Summary
Fructose and 2-deoxyglucose reduce phosphate release from mouse intestines by trapping phosphate during metabolism. This effect was partially reversed by certain compounds and influenced by inhibitors, suggesting a metabolic trapping mechanism in the gut wall.
Area of Science:
- Gastroenterology
- Cellular Metabolism
- Molecular Biology
Background:
- Phosphate transport is crucial for intestinal function.
- The metabolic fate of sugars can influence nutrient transport.
- Understanding sugar metabolism in the gut wall is key to nutrient absorption.
Purpose of the Study:
- To investigate the impact of fructose and 2-deoxyglucose on phosphate uptake and release in mouse intestinal sacs.
- To elucidate the mechanisms underlying the observed effects of these sugars on phosphate handling.
Main Methods:
- Utilized everted intestinal sacs from mice.
- Measured phosphate uptake and release in the presence of fructose and 2-deoxyglucose.
- Assessed the effects of metabolic modulators like succinate, fumarate, mannoheptulose, and adrenaline.
Main Results:
- Both fructose and 2-deoxyglucose significantly inhibited phosphate release without altering phosphate uptake.
- Succinate and fumarate partially reversed fructose-induced inhibition but not 2-deoxyglucose-induced inhibition.
- Mannnoheptulose (hexokinase inhibitor) improved phosphate release with both sugars.
- Adrenaline reduced the inhibitory effect of 2-deoxyglucose on phosphate release.
Conclusions:
- The inhibition of phosphate release by fructose and 2-deoxyglucose is likely due to the metabolic trapping of free phosphate within the gut wall.
- These findings highlight the role of sugar metabolism in regulating phosphate homeostasis in the intestine.