HYDRO: a program for protein hydropathy predictions

VAX/VMS programs for predicting protein hydropathy from amino acid sequence with several parameters are described. The hydropathy propensities of amino acids were normalised to make the different scales and predictions comparable. Another program package can be used to study similarity on the secondary structural level by comparing, for example, hydropathy predictions. These methods can provide valuable information also when there is only low sequence similarity. Amino acid residue accessibilities can be shown for known three-dimensional structures in addition to predictions. The programs can further be used for searching for a suitable prediction method and window size for a group of related proteins when the structure of at least one representative is known.