Related Experiment Video
Updated: Aug 8, 2026
09:01
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry
M Dardenne1, M do C de Moraes, P A Kelly
1CNRS URA 1461, Hôpital Necker, Paris, France.
Endocrinology
|May 1, 1994
Summary
Prolactin receptor (PRL-R) is widely expressed in human immune cells, including T-cells, B-cells, and monocytes. This suggests prolactin may regulate immune cell function through paracrine or autocrine signaling.
Area of Science:
- Immunology
- Endocrinology
Background:
- Prolactin receptor (PRL-R) plays a role in various physiological processes.
- Understanding PRL-R expression in hematopoietic tissues is crucial for immune system research.
Purpose of the Study:
- To analyze the expression of PRL receptor (PRL-R) in human hematopoietic tissues.
- To investigate the distribution and levels of PRL-R on different immune cell subsets.
Main Methods:
- Flow cytofluorometric analysis using biotinylated monoclonal antibodies against rat liver PRL-R.
- Analysis of T-cell subsets (CD4/CD8), B-cells, monocytes, and lymphoid cell lines.
Main Results:
- PRL-R is highly expressed in the thymus (>75% of cells).
- Ubiquitous PRL-R expression observed in peripheral blood immune cells (B-cells, monocytes, T-cells).
- PRL-R expression intensity increases in peripheral T-cells upon activation.
Conclusions:
- PRL-R is widely distributed across human hematopoietic cells.
- PRL signaling may influence immune cell function in both central and peripheral lymphoid organs.
- PRL gene expression in T-cells suggests autocrine/paracrine roles for prolactin in immunity.
