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Related Experiment Videos

Neuron-specific enolase expression during eye development in the chicken embryo

J Zwaan1, L Wang, A Garza

  • 1Department of Ophthalmology, University of Texas Health Science Center at San Antonio 78284-6230.

Experimental Eye Research
|January 1, 1994
PubMed
Summary

Neuron-specific enolase is not a reliable marker for neural crest-derived cells in the developing chicken eye, despite positive staining in neural tissues. Unexpectedly, it was found in the lens epithelium, suggesting a potential role similar to alpha-enolase.

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Area of Science:

  • Developmental biology
  • Neuroscience
  • Ophthalmology

Background:

  • Neuron-specific enolase (NSE) is a biomarker for neural crest cells in humans.
  • Neural crest contributions to ocular tissues are well-studied in chicken embryos.

Purpose of the Study:

  • To investigate the utility of NSE as a marker for neural crest-derived cells in the developing chicken eye.
  • To examine the expression pattern of NSE in ocular tissues during embryonic development.

Main Methods:

  • Immunohistochemical localization of NSE in chicken embryos at various developmental stages.
  • Utilized two distinct antisera specific for NSE.

Main Results:

  • Positive NSE staining observed in the neural retina nerve fiber layer and optic nerve starting around day 10.

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  • Ciliary ganglion also showed positive NSE staining.
  • No NSE staining detected in known neural crest-derived ocular tissues like cornea, sclera, trabecular meshwork, and iris stroma.
  • Moderate NSE activity detected in the lens epithelium from day 5 onwards.

    • Conclusions:

    • Neuron-specific enolase is not a reliable marker for identifying neural crest-derived cells in the chicken eye.
    • The presence of NSE in the lens epithelium suggests a potential role, possibly analogous to alpha-enolase, a known chicken lens crystallin.