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[Regulation of the complement C3 gene expression: regulatory elements required for cytokine-induced expression]
1Department of Pediatrics, Hokkaido University School of Medicine, Sapporo, Japan.
[Hokkaido Igaku Zasshi] the Hokkaido Journal of Medical Science
|March 1, 1994
Summary
This study identifies key DNA sequences regulating complement C3 gene expression in mice and humans. Specific elements control constitutive expression and responses to interleukin-1 and interleukin-6.
Area of Science:
- Molecular Biology
- Immunology
- Genetics
Context:
- Complement C3 is a crucial protein in the immune system.
- Understanding C3 gene regulation is vital for immune response research.
Purpose:
- To identify cis-acting regulatory elements in the 5'-flanking region of the complement C3 gene.
- To elucidate the molecular mechanisms controlling C3 gene expression in response to inflammatory signals.
Summary:
- Murine and human C3 gene 5'-flanking regions show significant homology, particularly in regulatory areas.
- A TATA box at -30 is essential for murine C3 expression in hepatocytes.
- Specific sequences (-395 to -111) are critical for constitutive C3 expression.
- Regulatory elements responsive to IL-1 and IL-6 were localized and linked to transcription factor binding sites (C/EBP, NF-kappa B).
Impact:
- Localizes cis-acting elements governing constitutive and cytokine-regulated C3 gene expression.
- Provides insights into the trans-acting factors that modulate C3 gene activity.
- Contributes to understanding the molecular basis of immune regulation and inflammation.