Analysis of magnetic resonance spectra by mole percent: comparison to absolute units

A variety of metabolites present in perchloric acid extracts of brain tissue were measured by 1H and 31P magnetic resonance spectroscopy (MRS) and HPLC in the same tissue sample and the MRS results were expressed both in terms of mole % and mumole/g based on an internal standard. The levels of 16 metabolites were compared by linear regression analysis and the mole % results were found to correlate very well with the results expressed as mumole/g. To compare the two units under typical experimental conditions, the percent change in metabolites in a group of Alzheimer's disease brains was compared to a control group using both units. The results were essentially identical for the mole % and mumole/g methods. We conclude that the use of the mole % method of expressing MRS data yields results which are equivalent to those expressed in absolute units and suggest that, for in vivo MRS studies, use of the mole % method is preferable because fewer artifacts, such as partial volume effects, are introduced.