[Preserved xenografts for the experimental replacement of bladder wall defects]

In 3 series of experiments on 62 rabbits the author tested feasibility of plastic correction of vast defects in the urinary bladder wall with human dura mater and pericardium pretreated with various proteolytic enzymes (papain, trypsin, chymotrypsin) of animal and plant origin. The levels of total protein were measured in the course of enzymatic proteolysis according to Lowry technique. The xenografts were studied biochemically prior to the proteolysis and after the treatment with proteolytic enzymes 6 times once an hour. The evidence was verified morphologically. Micromorphologically, the surface of the bladder was completely covered with connective tissue, the epithelium overgrew into some part of the transplant from the mucosa. Lecozyme was more effective in the pericardium, trypsin--in the dura mater. It is stated biochemically that optimal incubation time for proteolytic treatment of the xenograft is 3 hours.