Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparison of bone healing, as assessed by computed tomography, following tibial tuberosity advancement in dogs with and without autogenous cancellous bone grafts.

New Zealand veterinary journal·2017

Same author

Abnormal mismatch repair and other clinicopathologic predictors of poor response to progestin treatment in young women with endometrial complex atypical hyperplasia and well-differentiated endometrial adenocarcinoma: a consecutive case series.

BJOG : an international journal of obstetrics and gynaecology·2017

Same author

Negative pressure wound therapy using a portable single-use device for free skin grafts on the distal extremity in seven dogs.

Australian veterinary journal·2016

Same author

Major complications and risk factors associated with surgical correction of congenital medial patellar luxation in 124 dogs.

Veterinary and comparative orthopaedics and traumatology : V.C.O.T·2014

Same author

Foxc2 induces Wnt4 and Bmp4 expression during muscle regeneration and osteogenesis.

Cell death and differentiation·2013

Same author

Inter- and intra-operator variability associated with extracapsular suture tensioning: an ex vivo study.

Veterinary and comparative orthopaedics and traumatology : V.C.O.T·2012

Search research articles

Related Experiment Video

Updated: Jul 24, 2026

A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

Published on: December 6, 2014

Lymphoid-rich effusions. Diagnosis by morphometry using the CAS 200 System

A E Walts¹, R Svidler, T Tolmachoff

¹Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, California 90048.

American Journal of Clinical Pathology

|April 1, 1994

Summary

A computerized system accurately classified benign lymphocytosis and malignant lymphoma in effusions. This image analysis tool shows promise for improving diagnostic accuracy in clinical cytology.

More Related Videos

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Published on: January 9, 2019

Related Experiment Videos

Last Updated: Jul 24, 2026

A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

Published on: December 6, 2014

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Published on: January 9, 2019

Area of Science:

Cytopathology
Computational Pathology
Hematopathology

Background:

Lymphoid-rich effusions pose diagnostic challenges in clinical cytology.
Previous studies demonstrated the potential of computerized interactive morphometry for classifying these effusions.

Purpose of the Study:

To evaluate the efficacy of the CAS 200 System and an expert system for classifying lymphoid-rich effusions.
To assess the diagnostic accuracy for benign lymphocytosis, malignant lymphoma, and chronic lymphocytic leukemia.

Main Methods:

Analysis of 134 lymphoid-rich effusions (pleural, peritoneal, pericardial) using the CAS 200 System.
Application of criteria from a previously described rule-based expert system for classification.
Measurement of lymphoid nuclear profile images in Papanicolaou-stained cytospin smears.

Main Results:

The system achieved 98.9% accuracy for benign lymphocytoses and 88.9% for malignant lymphomas.
Predictive values of correct diagnoses were 95.7% for benign and 97.3% for malignant cases.
Chronic lymphocytic leukemias were not reliably distinguished from benign lymphocytoses based on nuclear profile areas.

Conclusions:

The CAS 200 System demonstrates high diagnostic accuracy for distinguishing benign lymphocytosis from malignant lymphoma in effusions.
Image analysis shows potential as a valuable tool in cytopathology for difficult effusion diagnoses.
Further investigation is needed for differentiating chronic lymphocytic leukemia using this methodology.