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A method to detect enteroviruses in sewage sludge-amended soil using the PCR
T M Straub1, I L Pepper, M Abbaszadegan
1Department of Soil and Water Science, University of Arizona, Tucson 85721.
Applied and Environmental Microbiology
|March 1, 1994
Abstract:
PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.