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Related Experiment Videos

Characterizing and sequencing cDNAs using oligonucleotide hybridization

W Bains1

  • 1PA Consulting Group, Melbourn, Royston, Herts, UK.

DNA Sequence : the Journal of DNA Sequencing and Mapping
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

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DNA sequencing using oligonucleotide hybridization can be improved by assuming the target DNA contains an open reading frame. This method extends sequencing capacity from 700 to 2400 bases, enabling substantial cDNA clone analysis.

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Oligonucleotide hybridization is a method for DNA sequencing.
  • Current methods face limitations in DNA length due to reconstruction ambiguity.

Purpose of the Study:

  • To enhance DNA sequencing capacity using oligonucleotide hybridization.
  • To overcome reconstruction ambiguity in DNA sequence determination.

Main Methods:

  • Utilizing a large panel of oligonucleotide probes for hybridization.
  • Constraining sequence reconstruction by assuming the presence of an open reading frame in the target DNA.
  • Employing mixed 11-mer probes for ideal hybridization scenarios.

Main Results:

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  • The DNA sequencing range was extended from 700 bases to 2400 bases.
  • This extension allows for the sequencing of substantial portions of cDNA clones.
  • Demonstrated practical potential for generating large-scale sequence data.

    • Conclusions:

    • Assuming an open reading frame significantly improves oligonucleotide hybridization-based DNA sequencing.
    • This approach offers a practical method for sequencing larger DNA fragments, particularly from cDNA libraries.