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Cellular retinoic acid-binding proteins (CRABP)

P Piletta¹, J H Saurat

¹Department of Dermatology, Hôpital Cantonal, University of Geneva, Switzerland.

Experimental Dermatology

|October 1, 1993

Summary

Mammalian cells have two retinoic acid (RA) binding proteins, CRABP I and CRABP II, which differ in their properties and functions. These proteins regulate intracellular RA levels, influencing its availability for nuclear receptors.

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Area of Science:

Molecular Biology
Cellular Biology
Biochemistry

Background:

Mammalian cells possess intracellular proteins that bind retinoic acid (RA).
Two primary retinoic acid binding proteins, cellular retinoic acid binding protein I (CRABP I) and CRABP II, have been identified.
These proteins are encoded by distinct genes and exhibit variations in their characteristics.

Purpose of the Study:

To elucidate the distinct properties and potential functional differences between CRABP I and CRABP II.
To understand the role of these binding proteins in regulating intracellular retinoic acid bioavailability.
To investigate how CRABP I and CRABP II modulate retinoic acid signaling pathways.

Main Methods:

Comparative analysis of CRABP I and CRABP II.
Assessment of RA binding affinities.
Investigation of transcriptional regulation by RA.
Examination of tissue distribution patterns.
Functional studies on intracellular RA metabolism.

Main Results:

CRABP I and CRABP II are produced from separate genes.
Significant differences exist in their affinity for retinoic acid.
Their transcriptional regulation by RA varies.
Distinct tissue distribution patterns were observed.
Potential functional divergence in regulating intracellular RA levels was suggested.

Conclusions:

CRABP I and CRABP II are distinct proteins with unique characteristics and likely specialized roles.
These proteins play a crucial role in the intracellular "intracine" metabolic process of retinoic acid.
They modulate the amount of biologically active retinoic acid available for nuclear receptor binding, thereby influencing gene transcription.

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