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Dominant lethals induced by Dithane M-45 in silkworm Bombyx mori
V Vasudev1, G Subramanya, N B Krishnamurthy
1Department of Studies in Zoology, University of Mysore, India.
Abstract:
Dithane M-45, a ethylenebis(dithiocarbamate) fungicide widely used in agriculture, including moriculture and sericulture, was tested for its efficacy in inducing dominant lethals in Bombyx mori. A polyvotine race of Pure Mysore was used for the studies and the topical application method was employed. After treatment with sublethal concentrations of 5, 10, and 20 g/50 larvae, the results have revealed that all of the concentrations could induce significant dominant lethals in a dose-dependent manner compared to controls. The results are discussed in the light of precautionary measures in the use of Dithane M-45 in the sericulture industry.
Insights
The fungicide Dithane M-45 induced significant dominant lethals in silkworms (Bombyx mori) at tested concentrations. This highlights potential risks for the sericulture industry, necessitating precautionary measures.
Area of Science:
- Toxicology
- Genetics
- Entomology
Background:
- Dithane M-45, an ethylenebis(dithiocarbamate) fungicide, is extensively used in agriculture.
- Its application extends to moriculture and sericulture, impacting silkworm production.
- Potential genotoxic effects of pesticides on non-target organisms are a growing concern.
Purpose of the Study:
- To evaluate the dominant lethal inducing potential of Dithane M-45 in the silkworm, Bombyx mori.
- To determine the dose-dependent genotoxic effects of this fungicide on silkworms.
- To inform safety protocols for Dithane M-45 use in sericulture.
Main Methods:
- The study utilized a polyvotine race of Pure Mysore silkworms.
- Topical application of sublethal Dithane M-45 concentrations (5, 10, 20 g/50 larvae) was performed.
- Control groups were maintained for comparative analysis.
Main Results:
- All tested concentrations of Dithane M-45 induced significant dominant lethals in Bombyx mori.
- The genotoxic effect was observed to be dose-dependent.
- Compared to controls, treated groups showed a statistically significant increase in dominant lethals.
Conclusions:
- Dithane M-45 exhibits genotoxic properties, inducing dominant lethals in silkworms.
- The findings underscore the need for stringent safety measures when using this fungicide in sericulture.
- Further research into safer alternatives or application methods is recommended.