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UV-responsive element (TGACAACA) from rat fibroblasts to human melanomas
1Molecular Carcinogenesis Program, American Health Foundation, Valhalla, New York 10595.
Environmental and Molecular Mutagenesis
|January 1, 1994
Summary
Proteins bound to the upstream regulatory element (URE) are linked to DNA replication and cell cycle progression. These URE-bound proteins may facilitate growth restoration after DNA damage.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- The upstream regulatory element (URE) is involved in gene regulation.
- Understanding the function of URE-bound proteins is crucial for comprehending DNA replication and cell cycle control.
Purpose of the Study:
- To investigate the association of URE-bound proteins with DNA replication.
- To explore the role of URE-bound proteins in cell cycle regulation and DNA damage response.
Main Methods:
- Analysis of URE-bound proteins' induction by UV and aphidicholin.
- Assessment of URE's ability to compete for factors promoting polyoma DNA replication.
- Examination of URE-bound protein expression during the S phase of the cell cycle.
Main Results:
- A 60 kDa trans-acting factor, potentially including URE-binding proteins, mediates polyoma DNA replication and is induced by UV.
- The URE competes for factors essential for polyoma replication in rodent cells.
- URE-bound proteins show increased expression during the S phase and are induced by aphidicholin, suggesting a role in growth restoration after DNA damage.
Conclusions:
- URE-bound proteins are associated with DNA replication and cell cycle progression.
- The URE may play a role in growth restoration following DNA damage.
- The growth release phenomenon is likely mediated by known and potentially unidentified transcription factors within the URE-bound protein complex.