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A negative regulatory element upstream from the mouse Adh-1 gene can down-regulate a heterologous promoter
M Yu1, R E Jerome, H J Edenberg
1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianopolis 46202-5122.
Abstract:
Regulation of Adh-1 (encoding mouse alcohol dehydrogenase-A2) gene expression results from the interplay of positive and negative effects. We report here the detection and localization of a negative regulatory element (NRE) to the sequence between -754 and -732 bp relative to the transcription start point (tsp). This element, which we call Adh-1 NRE2, can down-regulate transcription from a strong heterologous promoter (the SV40 promoter+enhancer). Adh-1 NRE2 can function in both orientations and either upstream or downstream from the promoter, and thus has attributes characteristic of a 'silencer'. A protein that binds to the Adh-1 NRE is widespread in mouse tissues.