Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CA 125 secretion by peritoneal mesothelial cells

A M Zeillemaker1, H A Verbrugh, A A Hoynck van Papendrecht

  • 1Department of Surgery, Diakonessen Hospital, Utrecht, The Netherlands.

Journal of Clinical Pathology
|March 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Goal Without a Plan Is Just a Wish-Creating a Personalized Aftercare Plan for Breast Cancer Patients Supported by the Breast Cancer Aftercare Decision Aid.

Current oncology (Toronto, Ont.)·2025
Same author

Excision of both pretreatment marked positive nodes and sentinel nodes improves axillary staging after neoadjuvant systemic therapy in breast cancer.

The British journal of surgery·2019
Same author

Cost-effectiveness analysis of Chlamydia trachomatis screening in Dutch pregnant women.

Pathogens and global health·2016
Same author

Discrimination of Aspergillus lentulus from Aspergillus fumigatus by Raman spectroscopy and MALDI-TOF MS.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2013
Same author

Frequency of microbiologically correct antibiotic therapy increased by infectious disease consultations and microbiological results.

Journal of clinical microbiology·2012
Same author

Course of murine tuberculosis and response to first-line therapy depends on route of infection and inoculum size.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2011

Human mesothelial cells constitutively secrete the tumor marker CA 125, primarily from their apical surface. Inflammatory cytokines like IL-1 beta and TNF-alpha, along with E. coli LPS, significantly enhance this CA 125 secretion.

Area of Science:

  • Cell Biology
  • Immunology
  • Biochemistry

Background:

  • CA 125 is a tumor marker often elevated in various conditions.
  • Mesothelial cells line serous cavities and play roles in inflammation and immunity.
  • Understanding CA 125 secretion by mesothelial cells is crucial for diagnostic and research purposes.

Purpose of the Study:

  • To investigate the secretion of CA 125 by cultured human mesothelial cells.
  • To determine if cytokine activation influences CA 125 secretion.
  • To analyze the polarization of CA 125 secretion in mesothelial monolayers.

Main Methods:

  • Human omentum-derived mesothelial cells were cultured to form confluent monolayers.
  • Monolayers were activated with recombinant interleukin-1 beta (rIL-1 beta), tumor necrosis factor-alpha (TNF-alpha), or Escherichia coli lipopolysaccharide (LPS).

Related Experiment Videos

  • Apical and basolateral CA 125 secretion was measured using a microparticle enzyme immunoassay.

    • Main Results:

    • Cultured mesothelial cells exhibited polarized CA 125 secretion, with a preference for the apical side.
    • Constitutive production of CA 125 was observed in non-activated mesothelial monolayers.
    • Incubation with rIL-1 beta, TNF-alpha, and E. coli LPS significantly enhanced CA 125 production.

    Conclusions:

    • Human mesothelial cells secrete CA 125 preferentially from their apical surfaces.
    • The secretion of CA 125 by mesothelial cells is significantly enhanced by inflammatory cytokines and bacterial components.
    • These findings contribute to understanding CA 125 regulation in physiological and pathological conditions.