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Related Experiment Videos

Small cortical thymocytes are subject to positive selection

K Lundberg1, K Shortman

  • 1Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.

The Journal of Experimental Medicine
|May 1, 1994
PubMed
Summary

Positive selection of T cells can occur in immature CD4+8+3lo thymocytes, not just mature ones. Enhancing cell survival increases the efficiency of this process in early T cell development.

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Area of Science:

  • Immunology
  • Developmental Biology
  • Cell Biology

Background:

  • T cell development involves positive selection in the thymus.
  • Previous studies suggested positive selection primarily acts on later-stage thymocytes.

Purpose of the Study:

  • To identify the specific developmental stages where positive selection can generate mature T cells.
  • To investigate the role of early thymocyte populations in T cell maturation.

Main Methods:

  • Sorting and transferring CD4+8+3lo thymocytes (large dividing and small non-dividing types) into recipient mice.
  • Utilizing transgenic mice with bcl-2/anti-TCR alpha beta to enhance cell survival.
  • Analyzing the production of mature CD4+8-3hi and CD4-8+3hi T cells.

Main Results:

  • Both small and large CD4+8+3lo thymocytes produced mature T cells, with smaller cells being less efficient.
  • Enhancing cell survival with bcl-2/anti-TCR improved the efficiency of small cell maturation.
  • Transgenic TCR expression confirmed small thymocytes' capacity for selection and maturation in a new environment.

Conclusions:

  • The developmental window for positive selection extends to include small CD4+8+3lo thymocytes.
  • CD4-8+ lineage T cells mature later than CD4-8+ lineage T cells due to intermediate stages.
  • Early thymocyte populations are capable of undergoing positive selection and contributing to mature T cell output.

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