[Analysis of synthetic corticoids by high performance liquid chromatography]

High performance liquid chromatography (HPLC) has been developed to analyze the synthetic corticoids (SCS) in human biological fluids. First, steroid moiety was extracted with a chemical solvent and/or an octadecylsilan (or diatomaceous earth) column, then the extracts were separated on the ordinary or reversed phase HPLC column with ultraviolet detection, at 254 nm. The total automatic system for the HPLC method has improved the accuracy and precision of SCS measurement for clinical use. Protein binding and free steroids, assayed by HPLC in plasma and saliva of the subjects treated with SCS may relate to steroid sensitivity. On the other hand, the simultaneous analysis of the metabolites of SCS in urine and other fluids reveals the clinical activity of the given SCS. HPLC analysis can therefore be utilized for evaluation of SCS pharmacodynamics.