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Decrease of hypochlorous acid and hydroxyl radical generated by stimulated human neutrophils: comparison in vitro of
B Gressier1, A Cabanis, S Lebegue
1Faculté des Sciences Pharmaceutiques et Biologiques, Laboratoire de Pharmacologie, Pharmacocinétique et Pharmacie Clinique, Lille, France.
Methods and Findings in Experimental and Clinical Pharmacology
|January 1, 1994
Summary
Two thiol drugs, mesna and N-acetylcysteine, effectively neutralize harmful reactive oxygen species like hypochlorous acid and hydroxyl radical, offering potential antioxidant therapy for inflammatory diseases.
Area of Science:
- Biochemistry
- Pharmacology
- Immunology
Background:
- Human neutrophils generate reactive oxygen species (ROS) during inflammation.
- Hypochlorous acid (HOCl) and hydroxyl radical (•OH) are key ROS implicated in diseases like arteriosclerosis and emphysema.
Purpose of the Study:
- To evaluate the in vitro antioxidant efficacy of mesna and N-acetylcysteine against neutrophil-derived ROS.
- To determine the potential of these drugs as therapeutic antioxidants.
Main Methods:
- In vitro assessment of antioxidant properties of mesna and N-acetylcysteine.
- Quantification of 50% inhibitory concentrations (IC50s) for HOCl and •OH scavenging by stimulated neutrophils.
Main Results:
- Mesna and N-acetylcysteine demonstrated significant antioxidant activity against HOCl (IC50s: 29 and 30 µM, respectively).
- Both drugs also inhibited hydroxyl radical production (IC50s: 520 and 480 µM, respectively).
Conclusions:
- Mesna and N-acetylcysteine effectively scavenge neutrophil-derived HOCl and •OH in vitro.
- These findings support their consideration as therapeutic agents to mitigate tissue damage in inflammatory conditions.