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Related Experiment Videos

Cytoskeletal changes associated with cell motility

R Warn1, D Brown, P Dowrick

  • 1School of Biological Sciences, University of East Anglia, Norwich, UK.

Symposia of the Society for Experimental Biology
|January 1, 1993
PubMed
Summary
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Cell motility involves rapid actin-rich ruffles and dynamic microtubules. Microtubule roles in cell polarity vary, with longer-lived microtubules identified in motile epithelial cells.

Area of Science:

  • Cell Biology
  • Cytoskeletal Dynamics
  • Cell Motility

Background:

  • Cellular activation into a motile phenotype involves significant cytoskeletal reorganization.
  • Actin-rich ruffles and microtubule dynamics are key features of cell movement.

Purpose of the Study:

  • To review cytoskeletal organization changes during the activation of cell motility.
  • To examine the formation of actin-rich ruffles and the role of microtubules in cell polarity.

Main Methods:

  • Observation of F-actin-rich ruffle formation upon addition of motility factors.
  • Micro-injection of biotin-tubulin to study microtubule turnover.
  • Comparison of microtubule dynamics in chick heart fibroblasts and epithelial cells.

Main Results:

Related Experiment Videos

  • Actin-rich ruffles form rapidly on cell surfaces, associated with increased pinocytosis.
  • Microtubule networks in chick heart fibroblasts show rapid turnover, irrespective of motility requirement.
  • A sub-population of long-lived microtubules was identified in motile epithelial cells, maintaining cell processes.

Conclusions:

  • Early markers of cell motility include rapid ruffle formation and associated pinocytosis.
  • Microtubule dynamics and their role in maintaining cell polarity are cell-type specific.
  • Long-lived microtubules may be crucial for maintaining cell shape and polarity in certain motile cell types.