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Related Experiment Videos

Actin and cell movement

J V Small1, A Rohlfs, M Herzog

  • 1Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.

Symposia of the Society for Experimental Biology
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

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Lamellipodial protrusion in eukaryotic cells is driven by actin polymerization, not gel swelling or network breakdown. This study supports the treadmilling model for actin dynamics in fish keratocytes.

Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics
  • Molecular Motors

Background:

  • Lamellipodia are key for eukaryotic cell locomotion.
  • Actin polymerization is a proposed mechanism for lamellipodia formation.
  • Alternative models like gel swelling and actin network breakdown have been suggested.

Purpose of the Study:

  • To investigate the mechanism of lamellipodial protrusion.
  • To differentiate between proposed models of actin dynamics in lamellipodia.
  • To analyze the ultrastructure of the keratocyte cytoskeleton.

Main Methods:

  • Combined videomicroscopy and electron microscopy of mouse macrophages.
  • Investigated the ultrastructure of fish keratocyte cytoskeleton.

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Main Results:

  • Lamellipodial protrusion in macrophages is linked to dense actin meshwork growth.
  • Gel swelling and cortical breakdown models were not supported.
  • Fish keratocyte lamellipodia contain long, regularly organized actin filaments, supporting treadmilling.

Conclusions:

  • Actin polymerization and treadmilling are key to lamellipodial protrusion.
  • The nucleation release model for keratocytes is not supported by ultrastructural data.
  • Lamellipodial dynamics involve organized, long actin filaments.