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FISH for Pre-implantation Genetic Diagnosis
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FISH for Pre-implantation Genetic Diagnosis

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De novo concurrent 5p deletion and distal 17q duplication identified by fluorescence in situ hybridization (FISH)

K Szego1, M Rauer, E Baratta

  • 1Rush Medical Laboratories, Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL 60612.

Annales De Genetique
|January 1, 1993
PubMed

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FISH - Fluorescent In-situ Hybridization02:07

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...