Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparative interphase cytogenetics using FISH on human ovarian carcinomas

T Liehr1, A Stübinger, K Thoma

  • 1Institute of Human Genetics, University of Erlangen-Nürnberg Schwabachanlage, Germany.

Anticancer Research
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Somatic homozygous loss of SH2B3, and a non-Robertsonian translocation t(15;21)(q25.3;q22.1) with NTRK3 rearrangement, in an adolescent with progenitor B-cell acute lymphoblastic leukemia with the iAMP21.

Cancer genetics·2022
Same author

A toddler with phylloid-type pigmentary mosaicism and ambiguous genitalia resulting from trisomy 14 induced by a der(Y)t(Y;14).

Human genome variation·2020
Same author

Chromosomal breakpoints in a cohort of head and neck squamous cell carcinoma patients.

Genomics·2019
Same author

BENIGN AND PATHOLOGICAL GAIN OR LOSS OF GENETIC MATERIAL — ABOUT MICROSCOPIC AND SUBMICROSCOPIC COPY NUMBER VARIATIONS (CNVs) IN HUMAN GENETICS.

Tsitologiia·2018
Same author

A new clinical variant of acquired reactive perforating dermatosis-like bullous pemphigoid.

The British journal of dermatology·2018
Same author

7q11.23 microduplication syndrome: neurophysiological and neuroradiological insights into a rare chromosomal disorder.

Journal of intellectual disability research : JIDR·2017
Same journal

Corrigendum.

Anticancer research·2026
Same journal

Geographic Variation in Stage at Diagnosis of Skin Cancer in the United States: A National Cancer Database Analysis.

Anticancer research·2026
Same journal

Anti-tumor Effects of Loureirin A Treatment in Colorectal Cancer Cells <i>via</i> Inhibition of AKT Phosphorylation.

Anticancer research·2026
Same journal

Bayesian Analysis to Refine East Asian Subgroup Estimates in the CLEAR Trial (Lenvatinib Plus Pembrolizumab <i>vs</i>. Sunitinib) for Advanced Renal Cell Carcinoma.

Anticancer research·2026
Same journal

Pulsed Electromagnetic Field Increases Doxorubicin-induced Mitotic Slippage in MDA-MB-231 Breast Cancer Cells.

Anticancer research·2026
Same journal

Geographic Disparities in Socioeconomic Factors and Advanced-stage Presentation in Rectosigmoid Cancer: A National Cancer Database Analysis.

Anticancer research·2026
See all related articles

Fluorescence in situ hybridization (FISH) revealed significant chromosome number changes in ovarian carcinomas. Specific losses of chromosomes 17 and 20, and gains of chromosomes 1, 7, 8, and 11 were common findings.

Area of Science:

  • Cytogenetics
  • Molecular Biology
  • Oncology

Background:

  • Ovarian carcinomas exhibit complex genetic alterations.
  • Understanding chromosomal abnormalities is crucial for diagnosis and treatment.

Purpose of the Study:

  • To investigate numerical chromosome abnormalities in human ovarian carcinomas using fluorescence in situ hybridization (FISH).
  • To identify specific chromosomal gains and losses associated with ovarian cancer.

Main Methods:

  • FISH analysis was performed on cytogenetic slides from 25 human ovarian carcinomas.
  • Biotinylated alphoid DNA probes were used for detecting chromosomes #1, #7, #8, #11, #12, #17, and #20 in interphase nuclei.

Main Results:

  • Significant findings included the specific loss of chromosomes #17 and #20 in aberrant cell populations.

Related Experiment Videos

  • Gains of chromosomes #7, #1, #8, and #11 were also frequently observed.
  • FISH demonstrated combined gains and losses of chromosomes within the same cell subpopulations, highlighting tumor heterogeneity.

    • Conclusions:

    • FISH is a powerful tool for interphase cytogenetics in analyzing ovarian carcinomas.
    • The study identified specific numerical chromosome abnormalities characteristic of ovarian cancer.
    • Conscientious consideration of FISH limitations is essential for accurate interpretation.