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The chromosome periphery during mitosis

D Hernandez-Verdun1, T Gautier

  • 1Institut Jacques Monod, Paris, France.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|March 1, 1994
PubMed
Summary
This summary is machine-generated.

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A distinct perichromosomal layer surrounds chromosomes during mitosis, composed of nuclear and nucleolar proteins and ribonucleoproteins. This layer plays roles in chromosome organization and cellular compartmentation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • A complex structure, the perichromosomal layer, is observed surrounding chromosomes during mitosis.
  • Its organization differs from chromosomes and cytoplasm, forming a distinct layer.
  • This layer can be isolated with chromosomes and covers them except at centromeric regions.

Purpose of the Study:

  • To characterize the composition and formation of the perichromosomal layer.
  • To elucidate the roles of the perichromosomal layer in cellular processes.

Main Methods:

  • Electron microscopy to visualize the perichromosomal layer.
  • Isolation of the perichromosomal layer with chromosomes.
  • Identification of proteins and RNAs within the layer.

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Main Results:

  • The perichromosomal layer comprises nuclear matrix proteins, nucleolar proteins, ribosomal proteins, and snRNAs.
  • Proteins extend from nucleoli during prophase and associate with decondensed chromatin during telophase.
  • The layer is involved in chromosome organization, acts as a barrier, aids in cell compartmentation, and binds chromosomal passenger proteins.

Conclusions:

  • The perichromosomal layer is a dynamic structure with diverse protein and RNP components.
  • It plays crucial roles in chromosome management and cellular organization during mitosis.