Isolation of three novel POU-domain containing cDNA clones from lactating mouse mammary gland

Using a PCR-based cloning strategy to identify putative members of the POU-family of transcription factors from genomic mouse DNA or from cDNA derived from lactating or involuting mouse mammary gland 56 POU-domain containing DNA fragments were isolated. Within these 56 clones three cDNA clones seem to be novel putative members of this transcription factor family, referred to as mPit-1R, mBrn-3R and MM-POU-III-A. Expression pattern studies were performed using a reverse transcriptase-mediated PCR approach. For all three different clones distinct developmental and tissue specific transcript levels were obtained, suggesting a tissue specific function of these newly isolated putative members of the POU-family of transcription factors.