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Updated: Aug 15, 2026
08:05
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
Published on: December 12, 2011
[Crossed immunoelectrophoresis in the serological diagnosis of amebiasis]
G.E.N
|January 1, 1975
Abstract:
Sixty-two patients were divided into two groups, and their sera was analyzed. a) In the first series, a study was made of the sera of patients with hepatic abscess wherein tests were positive in 100% of the cases. b) In the second serie, a study was made of the sera of patients with evidence of amoebiasis wherein tests were negative in 100% of the cases. Evaluation and diagnoses of patients were performed on the basis of clinical, radiological, scintillogram, coprological and endoscopic examinations.
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Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
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01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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01:28Amebiasis
Entamoeba histolytica, a protozoan parasite, is responsible for intestinal and extraintestinal amebiasis. Though a significant proportion of infections remain asymptomatic, approximately 50 million individuals annually are estimated to present with clinical disease, resulting in up to 100,000 deaths globally. The disease burden is disproportionately high in regions with lower socioeconomic status, such as parts of India, Africa, Mexico, and Latin America.Etiology and TransmissionThe infective...