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A sensitive fluorogenic enzyme linked immunosorbent assay for the detection of Vipera russelli venom
A R Bhatti1, J P Wong, Y M Siddiqui
1Defence Research Establishment Suffield, Department of National Defence, Medicine Hat, Alberta, Canada.
Natural Toxins
|January 1, 1993
Abstract:
A highly sensitive fluorogenic enzyme-linked immunosorbent assay (FELISA) has been adapted for the detection of Vipera russelli venom. The assay sensitivity was observed to be 0.1 pg/ml (1 x 10(-13) g/ml). Venoms from snakes of the Vipera group exhibited a high degree of cross-reactivity when tested with the antibody raised against V. russelli venom. With the exception of venom from Naja naja, all the tested venoms from unrelated families also showed cross-reactivity. This procedure is useful for detecting snake venom or its components in biological samples.