[Immunohistochemical localization of the epidermal growth factor receptor in mouse testes]

Immunohistochemical localization of the epidermal growth factor (EGF) receptor in mouse testes was studied. The biotin-avidin DCS system indirect immunofluorescent technique was used with mouse anti-human A431 cell EGF receptor monoclonal antibody 176 in vivo. Punctate fluorescence was detected in the cytoplasm of spermatogonia and Sertoli cells in the seminiferous tubules, while no fluorescence was observed in other seminiferous epithelial cells. Strong fluorescence was seen in Sertoli cells in vitro. These results suggest that spermatogonia and Sertoli cells are the target cells of epidermal growth factor.