Related Experiment Video
Updated: Aug 17, 2026

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
Published on: January 31, 2014
[Effect of IH764-3 on prolyl hydroxylation in collagen biosynthesis]
1Institute of Hematology, CAMS, Tianjin.
Abstract:
Previous study showed that IH764-3 selectively inhibited the synthesis and secretion of collagen, suggesting that the effect of the drug might be due to inhibition of prolyl hydroxylation. In this paper, we report the effect of IH764-3 on prolyl hydroxylation in collagen biosynthesis. Our results showed that the protocollagen substrate (Pro-Pro-Gly)10 9H2O of prolyl hydroxylation by proline hydroxylase isolated from 13-day-old chick embryos in the presence of a-ketoglutarate, ascorbic acid and Fe2+ was inhibited by IH764-3. The inhibition rate was about 50% at a concentration of 0.24 mmol/L. Further investigation demonstrated that IH764-3 did not bind to prolyl hydroxylase, and the inhibition of enzymatic prolyl hydroxylation was found to be due to chelation of the Fe2+ required for the enzymatic reaction. The molar ratio of Fe2+ to IH764-3 (1:3) was determined by the equilibrium movement method, and the chelate was postulated to be a octahedral complex.
Related Concept Videos
Structural Protein Function
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Protein Modifications in the RER
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.
Proteoglycans
Collagens are the Major Structural Proteins of ECM
Connective tissue proper includes loose...
Fibril-associated Collagen
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
Type IV Collagen of Basal Lamina
A type IV collagen molecule has six alpha chains which can exist in...

