Binding of the lipid peroxidation product 4-hydroxynonenal to human polymorphonuclear leukocytes
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Updated: Nov 9, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
M Curzio1, C Ferretti, R J Stephens
1Department of Experimental Medicine and Oncology, University of Turin, Italy.
Abstract:
4-Hydroxynonenal (HNE) is produced during peroxidation of polyunsaturated fatty acids. It exerts a chemokinetic effect on human polymorphonuclear leukocytes (PMN). Investigations of this mechanism were performed. The results indicate that [3H]-HNE binding to PMN results both in non-specific bonds to the numerous SH groups of the cells and in binding to a saturable, reversible and specific HNE site. Scatchard analysis revealed that this is a single site with an apparent affinity constant of 319 nM and a density of 1.57 pmol (10(6))-1 cells. This specific binding site may be involved in the chemokinetic effect of HNE.
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