Second messenger signalling in olfaction

Odorous molecules are recognized by specific receptor proteins located in the ciliary membrane of olfactory receptor neurons. These receptors have been identified using molecular cloning--they are members of the seven-transmembrane-domain G protein-coupled receptor superfamily. Specific receptor subtypes are expressed in subsets of olfactory neurons spatially segregated within certain areas of the olfactory epithelium. Interaction of odorants with receptors initiates the primary reaction of olfactory signalling. Intracellular reaction cascades are activated via specific G proteins, leading to a rapid and transient rise in second messenger levels; odorous compounds elicit mutually exclusive cAMP or inositol 1,4,5-trisphosphate responses. Odorant-induced second messenger signalling is terminated via kinase-mediated negative feedback loops uncoupling the reaction cascades by phosphorylation of receptor proteins. Strong odour stimuli elicit a delayed response of another messenger system, the nitric oxide/cGMP cascade. cGMP may control some adaptive reactions in olfactory receptor neurons.