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Mouse sialoadhesin is not responsible for Candida albicans yeast cell binding to splenic marginal zone macrophages
Y Han1, S Kelm, M H Riesselman
1Department of Microbiology, Montana State University, Bozeman 59717.
Abstract:
Candida albicans hydrophilic yeast cells specifically adhere to mouse macrophages in the splenic marginal zone and in lymph node subcapsular and medullary sinuses. These macrophages express sialoadhesin that binds erythrocytes, but binding of yeast cells is not mediated by sialoadhesin because (i) erythrocytes did not block yeast cell binding, (ii) yeast cell adherence was unaffected by sialoadhesin-specific monoclonal antibodies, (iii) the purified sialoadhesin did not bind to yeast cells, and (iv) an Fc-sialoadhesin chimera immobilized on plastic did not bind yeast cells. These data give further support for a unique macrophage adhesion system that binds C. albicans.
Insights
Candida albicans yeast cells bind to specific mouse macrophages. This interaction is not mediated by sialoadhesin, suggesting a unique macrophage adhesion system for C. albicans.
Area of Science:
- Immunology
- Microbiology
- Cell Biology
Background:
- Candida albicans is a common fungal pathogen.
- Macrophages play a crucial role in innate immunity against fungal infections.
- Specific adhesion mechanisms are vital for host-pathogen interactions.
Purpose of the Study:
- To investigate the mechanism of Candida albicans yeast cell adhesion to mouse macrophages.
- To determine if sialoadhesin is involved in the binding of C. albicans to macrophages.
Main Methods:
- Microscopy to observe yeast cell adherence to macrophages in splenic marginal zones and lymph nodes.
- Inhibition assays using erythrocytes and sialoadhesin-specific monoclonal antibodies.
- Binding assays with purified sialoadhesin and an Fc-sialoadhesin chimera.
Main Results:
- Candida albicans yeast cells specifically adhered to mouse macrophages in the spleen and lymph nodes.
- Erythrocytes did not block yeast cell binding to macrophages.
- Sialoadhesin-specific antibodies did not affect yeast cell adherence.
- Purified sialoadhesin and an Fc-sialoadhesin chimera did not bind to yeast cells.
Conclusions:
- Sialoadhesin does not mediate the binding of Candida albicans yeast cells to mouse macrophages.
- These findings support the existence of a unique macrophage adhesion system for C. albicans.