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Effect of glucosamine on virus production and antigen expression in avian sarcoma virus-transformed cells

E Israel, R Mandeville, M A Wainberg

    Canadian Journal of Microbiology
    |September 1, 1978
    PubMed

    Insights

    Glucosamine treatment halts avian sarcoma virus replication in chicken cells, preventing infectious virus production. However, non-infectious viral particles can still form, and glucosamine impacts cell antigen expression.

    Area of Science:

    • Virology
    • Cell Biology
    • Immunology

    Background:

    • Avian sarcoma virus transforms chicken embryo fibroblast (CEF) cells.
    • Viral particle synthesis and infectivity are key aspects of viral replication.
    • Cellular immune responses and antigen expression are crucial in viral infections.

    Purpose of the Study:

    • To investigate the effect of glucosamine on avian sarcoma virus replication in CEF cells.
    • To determine if glucosamine affects the production of infectious viral particles.
    • To assess the impact of glucosamine on antigenic expression and cell-mediated immunity in transformed CEF cells.

    Main Methods:

    • Treatment of CEF cells with glucosamine.
    • Analysis of progeny-transforming virus particle formation.
    • Detection of non-infectious physical particles containing viral RNA and RNA-dependent DNA polymerase.
    • Indirect immunofluorescence assay for antigenic expression.
    • Lymphocyte stimulation assay for cell-mediated immunity.

    Main Results:

    • Glucosamine completely inhibited the formation of progeny-transforming virus particles.
    • Non-infectious physical particles containing viral RNA and RNA-dependent DNA polymerase were synthesized when glucose was present during glucosamine exposure.
    • Glucosamine treatment affected antigenic expression in transformed CEF cells.
    • A lesser inhibition was observed in a lymphocyte stimulation assay for cell-mediated immunity.

    Conclusions:

    • Glucosamine is a potent inhibitor of infectious avian sarcoma virus production.
    • Glucosamine's effect on viral particle synthesis is dependent on the presence of glucose.
    • Glucosamine influences the antigenic profile of transformed CEF cells and has a limited impact on cell-mediated immunity.

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